منابع مشابه
Human-derived alternatives to fetal bovine serum in cell culture.
OBJECTIVE The need for an alternative to fetal bovine serum (FBS) is known to scientists and users involved in cell therapy or advanced therapy medicinal products. Human serum (huS) and platelet lysate (hPL) can be used as alternatives resulting in similar or even superior results concerning cell expansion. METHODS We developed protocols for the production of huS and two types of hPL and test...
متن کاملAlternatives to the use of fetal bovine serum: serum-free cell culture.
Serum is commonly used as a supplement to cell culture media. It provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors. The most widely used animal serum supplement is fetal bovine serum, FBS. Since serum in general is an ill-defined component in ce...
متن کاملMaking the Switch: Alternatives to Fetal Bovine Serum for Adipose-Derived Stromal Cell Expansion
Adipose-derived stromal cells (ASCs) are being used extensively in clinical trials. These trials require that ASCs are prepared using good manufacturing practices (GMPs) and are safe for use in humans. The majority of clinical trials in which ASCs are expanded make use of fetal bovine serum (FBS). While FBS is used traditionally in the research setting for in vitro expansion, it does carry the ...
متن کاملAlternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
The search for alternatives to the use of fetal bovine serum (FBS) in cell and tissue culture media has become a major goal in terms of the 3R principles in order to reduce or to avoid harvesting of FBS from bovine fetuses, and, in terms of Good Manufacturing Practice (GMP), to ensure safe and animal product-free conditions for biomedical tissue engineering and (adult) stem cell therapy, respec...
متن کاملFetal Bovine Serum (FBS): Past - Present - Future.
The supplementation of culture medium with fetal bovine serum (FBS, also referred to as "fetal calf serum") is still common practice in cell culture applications. Due to a number of disadvantages in terms of quality and reproducibility of in vitro data, animal welfare concerns, and in light of recent cases of fraudulent marketing, the search for alternatives and the development of serum-free me...
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ژورنال
عنوان ژورنال: Nature Biotechnology
سال: 1995
ISSN: 1087-0156,1546-1696
DOI: 10.1038/nbt0495-338